Main objective
To analyze EBOV persistence in body fluids (semen or vaginal secretions/menstrual blood/breast milk/rectal swabs/saliva/sweat/urine/tears) in relation to immune responses.

To assess concordance between RT-PCR test results and virus isolation test results

Pilot: Aims to assess persistence of EBOV in semen among a small cohort of male survivors including assessing the feasibility of the data collection and collection and testing of semen.

Main study: Aims to assess persistence of EBOV in semen, vaginal secretions, and other body fluids (i.e. rectal, sweat, urine, saliva, tears, menstrual blood and breast milk, if any lactating women recruited) together with immune responses and HIV status among male and female EVD survivors in Sierra Leone.